Molecular Biology technical platform

SCIENTIFIC BACKGROUND AND OBJECTIVES

The aim of this technical platform is to pool technologies and skills in molecular biology to support the unit’s research programmes.

The methods used generate the genetic data needed to characterise biodiversity and study phylogeny and population genetics, as well as genome structure, organism adaptation and gene function for species or communities of interest.

The site has 13 permanent staff and welcomes a large number of students.

Anne-Laure CLAMENS

Technician, INRAE

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Anne-Laure CLAMENS

Technician, INRAE

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Sabine NIDELET

Technician, INRAE

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Sabine NIDELET

Technician, INRAE

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Rémi ALLIO

Research manager, INRAE

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Rémi ALLIO

Research manager, INRAE

Voir le profil

See the platform permanent members

Activities and main facilities available

The platform has 200 m² of laboratories dedicated to the various activities based on the principle of forward progress. The main activities are as follows:

High-throughput purification of nucleic acids (RNA and DNA)
Main equipment: Sigma 4K15 centrifuge, Eppendorf epMotion 5075 robot
PCR amplification for genotyping (microsatellites, RFLP, AFLP) and sequencing (conventional and high-throughput)
Main equipment: 96 Eppendorf block thermocyclers (Mastercycler, epGradient and Nexus) and 384 TProfessional (Biometra)
Quantitative PCR: measurement of gene expression, pathogen detection, target quantification
Main equipment: LightCycler 480 (Roche), 96- and 384-well blocks
Preparation of libraries for high-throughput sequencing: AmpliconSeq (metabarcoding, 16S metagenomics, MHC genotyping, etc.), RADseq, RNAseq, Shotgun, etc.
Quality control of nucleic acids, PCR products and genomic libraries
Main equipment: electrophoresis tanks (Mupid), Bioanalyzer 2100 (Agilent), NanoDrop ND8000 spectrophotometer (Thermo), Qubit fluorometer (Invitrogen)
Cytogenetics: karyotyping, PRINS, FISH of repeated probes, BAC-FISH, chromosome painting, Zoo-FISH
Main equipment: fluorescence microscope (Zeiss) coupled to an image analysis system (Genus, Cytovision)

As CBGP is part of the LabEx CeMEB (Centre Méditerranéen Environnement et Biodiversité) and the LabEx Agro, users of our technical platform also benefit from access to shared equipment on partner platforms (Applied Biosystems capillary sequencers, Illumina MiSeq sequencer, Thermofisher KingFisher automated extraction system, Covaris S220 sonicator):

CeMEB labelled platforms

Grand Regional Genotyping Technical Platform

Penel* B., Genty L., Marty C., Bourdonné A., Clamens A.-L., Benoit L., Soldati L., Migeon A., Kergoat G.J., Haran J., Fried G. & Meynard C. 2026. Beetle communities in agricultural landscapes: relative influences of climate, landscape, plant communities and agricultural practices. Agriculture, Ecosystems & Environment 400 : 110252. (https://dx.doi.org/10.1016/j.agee.2026.110252)

Haran J., Zelvelder* B., Archange Boupoya C., Couvreur T.L.P., Niangadouma R., Benoit L., Kergoat G.J., Buatois B., Dufäy M., Kojima H. & Allio R. 2026. New species of Endaeus Schoenherr, 1826 (Curculionidae: Curculioninae: Ochyromerini) associated with Annonaceae and Clusiaceae. European Journal of Taxonomy 1039 : 165–198. (https://dx.doi.org/10.5852/ejt.2026.1039.3185)

Ganem G., Dufour C.M.S., Avenant N.L., Berthier K., Loiseau A., Caminade P., Pillay N. & Brouat C. 2026. Rare interspecific hybridisation between sympatric African four-striped mice, and indications of fine-scale intraspecific spatial structure related to social factors. Journal of Vertebrate Biology 75 : 25073. (https://dx.doi.org/10.25225/jvb.25073)

Atteynine S.A., Bertrand M., Dembélé A., Coulibaly F., Diagne C. & Granjon L. 2026. Tempo and mode in biological invasions: exotic rodents in the small mammal community of Bamako (Mali). Mammalian Biology 106 : 129-146. (https://dx.doi.org/10.1007/s42991-025-00542-7)

Zhang Y.M., Delvare G., Blaimer B.B., Cruaud A., Rasplus J.-Y., Brady S.G. & Gates M.W. 2025. Phasing in and out of phytophagy: Phylogeny and evolution of the family Eurytomidae (Hymenoptera: Chalcidoidea) based on Ultraconserved Elements. Systematic Entomology 50 : 780-793. (https://dx.doi.org/10.1111/syen.12682)

Wieczorek K., Chlond D., Chajec L., Malik K., Swiatek P., Jaroszewicz J., Coulson S.J. & Jousselin E. 2025. Integrative approach to the systematics of the endemic Svalbard aphid specis Macrosiphum calvulum (Hemiptera, Aphididae) using molecular morphological and reproductive system analysis. Scientific Reports 15 : 26960. (https://dx.doi.org/10.1038/s41598-025-12913-8)